To test whether gluten was transferred by kissing, first, the partner without celiac disease ate 10 crackers. Then, they waited 5 minutes, or they drank 4 ounces of water. Next, the couples were instructed to kiss with an open mouth for at least one minute, involving the tongue and saliva transfer.
Saliva was then collected from the partner with celiac disease, and their urine was tested for gluten absorption.